Nikola Hudáková, Mag. pharm.
position: full-time doctoral student (2020 – 2024)
Dissertation topic: Study of the influence of the inflammatory environment on the regenerative potential of stem cells and their metabolites
As part of the dissertation, the focus is on the standardization of isolation, cultivation and freezing protocols for mesenchymal stromal perivascular stem cells (human and canine) isolated from neonatal and adult tissues and their characterization, on the optimization of the inflammatory environment in vitro for the purpose of the highest immunomodulating and regenerative effect of selected mesenchymal stem cells, for the analysis of anti-inflammatory, neurotrophic and regenerative molecules in the conditioned media of mesenchymal stem cells and for functional studies of their effects on cell proliferation and apoptosis in an inflammatory environment. Demonstration of neuroregenerative efficacy opens up the possibility of translation into preclinical studies of neurodegenerative diseases.
Juraj Vozár, D.V.M.
position: external doctoral student (2021 – 2026)
Dissertation topic: Study of the immunomodulating and regenerative properties of the eggshell membrane
During his doctoral studies, he is devoted to the characterization of membranes from eggshells separated and processed from egg waste in order to analyze the anti-inflammatory and regenerative properties of this material. Eggshell membrane is a natural biomaterial that is used as an alternative material for burns and skin injuries, but also for joint diseases. Monitoring the secretion of pro-inflammatory (IL-1 and IL-6) and anti-inflammatory cytokines (IL-10) will reveal a possible immunomodulating effect in an in vitro and in vivo model system. The influence of metalloproteases can have a positive effect on the regenerative properties of the extracellular matrix during wound healing and possibly other tissue diseases.
Michal Domaniža, D.V.M.
position: external doctoral student (2021 – 2026) – in cooperation with the Small animal clinic
Dissertation topic: Study of the therapeutic effect of mesenchymal stem cells on some disease processes of bones and joints
Graduated doctoral students
Zuzana Vikartovská, D.V.M., B.Sc., Ph.D.
Year of graduation: 2021
Dissertation topic: Adult stem cells in the treatment of cognitive dysfunctions in dogs
Filip Humeník, D.V.M. Ph.D.
Year of graduation: 2021
Dissertation topic: Alternative sources of adult stem cells and their products: multilineage and paracrine potential
- Angelos Costa: Stem cells in 2D and 3D culture system
- Ivana Ulbríková: Effect of conditioned medium of adult stem cells on proliferation (2022)
- Miroslava Hriňáková: Isolation and characterization of mesenchymal stem cells from the amnion of dogs (2022)
- Vanesa Pohánková: Changes in CD markers of adult MSCs depending on the length of cultivation (2022)
- Sonia Jego: Treatment of neurodegenerative disorders using stem cells (2021)
- Jessika El Rifiová: Cryopreservation of mesenchymal stem cells: advantages and disadvantages (2021)
The ambitions of the center are also transferred to the pedagogical field:
- a guarantee of the profile subjects “Neurogenesis and plasticity of the CNS” and “In vitro cell cultivation” for the 3rd degree of study in the field of Biology and the study program of Neuroscience and Regenerative Medicine for the 3rd degree of study in the field of Surgery.
- a guarantee of the mandatory optional subject “Basics of regenerative medicine” for the 3rd year of VVL and GVM
- education of graduates, internal and external doctoral students in the study field of Biology and in the study program of Neuroscience (till 2022).