{"id":33,"date":"2022-08-08T12:06:27","date_gmt":"2022-08-08T10:06:27","guid":{"rendered":"https:\/\/cekrm.uvlf.sk\/?page_id=33"},"modified":"2022-08-30T13:18:24","modified_gmt":"2022-08-30T11:18:24","slug":"methodologies","status":"publish","type":"page","link":"https:\/\/cekrm.uvlf.sk\/en\/methodologies\/","title":{"rendered":"Methodologies"},"content":{"rendered":"\r\n<p>The Centre of Experimental and Clinical Regenerative Medicine uses the following methodological procedures as part of its scientific and research activities:<\/p>\r\n\r\n\r\n\r\n<ul class=\"wp-block-list\">\r\n<li>isolation of mesenchymal stem cells from adult tissues (bone marrow, adipose tissue, liver and spleen) and neonatal tissues (umbilical cord, amnion and placenta),<\/li>\r\n<li>morphological and phenotypic characterization (flow cytometry) of isolated mesenchymal stem cells,<\/li>\r\n<li>multilineage characterization of mesenchymal stem cells,<\/li>\r\n<li>isolation of RNA and DNA from cultured cell populations,<\/li>\r\n<li>preparation of the conditioned medium of the monitored cell populations,<\/li>\r\n<li>isolation of primary cultures from different tissues (fibroblasts, keratinocytes, neural progenitors) of neural cells from the central nervous system,<\/li>\r\n<li>isolation of primary cultures of tumor cells from mammary gland carcinoma,<\/li>\r\n<li>immunocytochemical and immunohistochemical methods,<\/li>\r\n<li>application of stem cells and their products in regenerative medicine,<\/li>\r\n<li>identification of biomarkers for oncological diseases of the mammary gland,<\/li>\r\n<li>screening of biomarkers for neurodegenerative diseases.<\/li>\r\n<\/ul>\r\n","protected":false},"excerpt":{"rendered":"<p>The Centre of Experimental and Clinical Regenerative Medicine uses the following methodological procedures as part of its scientific and research activities: isolation of mesenchymal stem cells from adult tissues (bone marrow, adipose tissue, liver and spleen) and neonatal tissues (umbilical cord, amnion and placenta), morphological and phenotypic characterization (flow cytometry) of isolated mesenchymal stem cells, multilineage characterization of mesenchymal stem cells, isolation of RNA and DNA from cultured cell populations, preparation of the conditioned medium of the monitored cell populations, isolation of primary cultures from different tissues (fibroblasts, keratinocytes, neural [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page_fullwidth.php","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-33","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/cekrm.uvlf.sk\/en\/wp-json\/wp\/v2\/pages\/33"}],"collection":[{"href":"https:\/\/cekrm.uvlf.sk\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/cekrm.uvlf.sk\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/cekrm.uvlf.sk\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cekrm.uvlf.sk\/en\/wp-json\/wp\/v2\/comments?post=33"}],"version-history":[{"count":5,"href":"https:\/\/cekrm.uvlf.sk\/en\/wp-json\/wp\/v2\/pages\/33\/revisions"}],"predecessor-version":[{"id":323,"href":"https:\/\/cekrm.uvlf.sk\/en\/wp-json\/wp\/v2\/pages\/33\/revisions\/323"}],"wp:attachment":[{"href":"https:\/\/cekrm.uvlf.sk\/en\/wp-json\/wp\/v2\/media?parent=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}